Hemolysis Affects C-Peptide Immunoassay

Diagnostic performance of the ARCHITECT C-Peptide immunoassay.

BACKGROUND Measurement of C-peptide under standardized conditions provides a sensitive and well-established assessment of beta-cell function. We describe the analytical and clinical validation of an automated, microparticle-based chemiluminescent immunoassay method. The assay is designed to measure C-peptide in human serum, plasma and urine. METHODS Assay performance characteristics such as p...

Identification of proinsulin and C-peptide in human serum by a specific immunoassay.

An immunoassay for the measurement of human proinsulin and C-peptide is described. By a combination of this system and an insulin immunoassay, proinsulin, insulin, and C-peptide were identified in acid-ethanol extracts of serum after gel filtration. In keeping with the results of biosynthetic studies, insulin and C-peptide were found to be present in equimolar amounts. The absolute values of se...

[C-peptide].

Chemiluminescence immunoassay for somatomedin C in serum.

To select the best tracer for use in a competitive immunoassay, we conjugated human somatomedin C (SmC) to various chemiluminescent compounds via two different synthetic pathways. Naphthylhydrazides and arylhydrazides, used as the labels, were incorporated via their imidate or their succinimide esters. Conjugating the carboxy terminal of (amino ethyl)ethyl-isoluminol to SmC via a succinimide li...

Solid phase immunoassay for C trachomatis.

CORRESPONDENCE Solid phase immunoassay for C trachomatis The development of chlamydial antigen detection kits has led to the wider availability of diagnostic services for this organism. Currently, two technologies are readily available; immunofluorescence microscopy and enzyme linked immunosorbant assay (EIA). Neither technology is perfect, with problems of sensitivity and of specificity.' Adva...